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Cyclopropane fatty acids improve Escherichia coli survival in acidified minimal media by reducing membrane permeability to H+ and enhanced ability to extrude H+
journal contributionposted on 2023-05-16, 20:50 authored by Svetlana ShabalaSvetlana Shabala, Thomas RossThomas Ross
The physiological role of cyclopropane fatty acids (CFAs) in acid stress resistance was studied by in situ microelectrode H+ flux measurements of Escherichia coli Frag1 and its isogenic CFA-deficient mutant JBM 1. After exposure to pH 4 for 16 h, net H+ influx in JBM 1 was twice that of the parent strain. H+ flux stabilisation at pH 6 after acid stress took more time in the cfa- mutant. The data suggest increased proton permeability and decreased ability to extrude H+ in the absence of CFA, and they support the hypothesis that CFAs protect E. coli in acidic environments by decreasing membrane permeability to H+. Crown Copyright Â© 2008.
Publication titleResearch in Microbiology
Department/SchoolTasmanian Institute of Agriculture (TIA)
PublisherElsevier France, Editions Scientifiques et Medicales
Place of publicationFrance