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DNA as a dietary biomarker in Antarctic krill, Euphausia superba

journal contribution
posted on 2023-05-16, 18:33 authored by Passmore, A, Jarman, SN, Kerrie SwadlingKerrie Swadling, Kawaguchi, SR, Andrew McMinnAndrew McMinn, Nicol, S
The diet of Antarctic krill (Euphausia superba) has been studied using a variety of techniques, but current methods still suffer from problems that are difficult to solve. This study examined an alternative approach utilizing DNA as a prey biomarker. Methods were developed for the preservation, extraction, and identification of prey DNA from krill collected in the field. Group-specific polymerase chain reaction (PCR) was used to amplify diatom prey (Phylum: Bacillariophyta) and the results from DNA clone libraries were compared with microscopic diet analysis. DNA analysis was superior to microscopy for prey detection. However, differences in prey relative abundance estimates between the two techniques suggested some bias in the DNA-based estimates. Quantification showed that large amounts of prey DNA had been successfully preserved and extracted. Overall the results suggest that the application of DNA-based diet analysis to krill warrants further investigation, particularly for prey that are difficult to study using other methods.

History

Publication title

Marine Biotechnology

Volume

8

Issue

6

Pagination

686-696

ISSN

1436-2228

Department/School

Institute for Marine and Antarctic Studies

Publisher

Springer

Place of publication

NY, USA

Repository Status

  • Restricted

Socio-economic Objectives

Biodiversity in Antarctic and Southern Ocean environments

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