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Defective Long-Distance Auxin Transport Regulation in the Medicago truncatula super numeric nodules Mutant

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posted on 2023-05-16, 18:06 authored by van Noorden, GE, John RossJohn Ross, James ReidJames Reid, Rolfe, BG, Mathesius, U
Long-distance auxin transport was examined in Medicago truncatula and in its supernodulating mutant sunn (super numeric nodules) to investigate the regulation of auxin transport during autoregulation of nodulation (AON). A method was developed to monitor the transport of auxin from the shoot to the root in whole seedlings. Subsequently, the transport was monitored after inoculation of roots with the nodulating symbiont Sinorhizobium meliloti. The sunn mutant showed an increased amount of auxin transported from the shoot to the root compared to the wild type. The auxin transport capacity of excised root segments was similar in wild type and sunn, suggesting that the difference in long-distance auxin transfer between them is due to loading in the shoot. After inoculation, wild-type seedlings showed decreased auxin loading from the shoot to the root; however, the sunn mutant failed to reduce the amount of auxin loaded. The time of reduced auxin loading correlated with the onset of AON. Quantification of endogenous auxin levels at the site of nodule initiation showed that sunn contained three times more auxin than wild type. Inoculation of sunn failed to reduce the level of auxin within 24 h, as was observed in the wild type. We propose a model for the role of auxin during AON of indeterminate legumes: 1) high levels of endogenous auxin are correlated with increased numbers of nodules, 2) inoculation of roots reduces auxin loading from the shoot to the root, and 3) subsequent reduction of auxin levels in the root inhibits further nodule initiation. © 2006 American Society of Plant Biologists.

History

Publication title

Plant Physiology

Volume

140

Issue

4

Pagination

1494-1506

ISSN

0032-0889

Department/School

School of Natural Sciences

Publisher

American Society of Plant Biologists

Place of publication

United States

Repository Status

  • Restricted

Socio-economic Objectives

Environmentally sustainable plant production not elsewhere classified

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