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Defined medium conditions for the induction and expansion of human pluripotent stem cell-derived retinal pigment epithelium
journal contributionposted on 2023-05-18, 17:45 authored by Lidgerwood, GE, Lim, SY, Crombie, DE, Ali, R, Gill, KP, Hernandez, D, Kie, J, Conquest, A, Waugh, HS, Wong, RCB, Liang, HH, Alexander HewittAlexander Hewitt, Davidson, KC, Pebay, A
We demonstrate that a combination of Noggin, Dickkopf-1, Insulin Growth Factor 1 and basic Fibroblast Growth Factor, promotes the differentiation of human pluripotent stem cells into retinal pigment epithelium (RPE) cells. We describe an efficient one-step approach that allows the generation of RPE cells from both human embryonic stem cells and human induced pluripotent stem cells within 40â€“60 days without the need for manual excision, floating aggregates or imbedded cysts. Compared to methods that rely on spontaneous differentiation, our protocol results in faster differentiation into RPE cells. This pro-retinal culture medium promotes the growth of functional RPE cells that exhibit key characteristics of the RPE including pigmentation, polygonal morphology, expression of mature RPE markers, electrophysiological membrane potential and the ability to phagocytose photoreceptor outer segments. This protocol can be adapted for feeder, feeder-free and serum-free conditions. This method thereby provides a rapid and simplified production of RPE cells for downstream applications such as disease modelling and drug screening.
Publication titleStem Cell Reviews and Reports
Department/SchoolTasmanian School of Medicine
Place of publicationGermany
Rights statementCopyright 2015 Springer Science+Business Media New York