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Design and performance of a light-emitting diode detector compatible with a commercial capillary electrophoresis instrument

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posted on 2023-05-16, 15:35 authored by Johns, CA, Miroslav MackaMiroslav Macka, Paul HaddadPaul Haddad
Indirect photometric detection in capillary electrophoresis (CE) has been predominantly performed in the UV region, in part due to a lack of suitable high-intensity and low-noise light sources in the visible spectral region. A new photometric detector based on light-emitting diodes (LEDs) as light sources and compatible with a commercially available CE instrument has been designed and constructed and its performance evaluated. The utility of this detector was successfully demonstrated by the indirect photometric detection of anions using a dye as probe and absorbance measured in the visible region. The detector exhibited very low baseline noise (around 0.03 mAU), stable output, and improved upper limit of detection linearity (502 mAU) compared with previously used LED detectors. The detector was tested for indirect detection of anions separated with an electrolyte containing 4 mM Orange G as the indirect detection probe, 10 mM histidine as an isoelectric buffer, and 0.05% hydroxypropylmethylcellulose to suppress the electroosmotic flow. Extremely low detection limits were obtained ranging from 0.16-0.36 μM (excluding chloride 0.56 μM), with separation efficiencies in the range of 154 000-274 000 theoretical plates. © 2004 Wiley-VCH Verlag GmbH & Co. KGaA.

History

Publication title

Electrophoresis

Volume

25

Issue

18-19

Pagination

3145-3152

ISSN

0173-0835

Department/School

School of Natural Sciences

Publisher

Wiley-VCH

Place of publication

Weinheim

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the chemical sciences

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