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Detection of the Ability of Tricholoma matsutake to Utilize Sawdust in Aseptic Culture
journal contributionposted on 2023-05-16, 14:16 authored by Vaario, LM, Warwick GillWarwick Gill, Samejima, M, Suzuki, K
Four isolates of Tricholoma matsutake were investigated for growth on media supplemented with combinations of glucose and sawdust. After 5 weeks of incubation, the transmission electron microscope observations indicated that hyphae of T. matsutake (T945) hyphae were able to degrade the xylem cell walls of sawdust in a sawdust-containing medium. After 7 weeks of incubation, the quantity of ergosterol produced by three isolates (T945, T61, and Y1) was significantly greater on the medium containing sawdust than the medium without. The activity of p-nitrophenyl-Î²-D-lactopyranosidase, a cellulolytyic enzyme, was monitored in T. matsutake cultures as a presumptive assay for extracellular cellulolytic activity. p-Nitrophenyl-Î²-D-lactopyranosidase (p-NPL) activity of T945 was significantly higher on sawdust containing media containing sawdust and 0.1 g/l glucose than on other media. It is suggested that isolate T945 of T. matsutake is capable of utilizing sawdust in aseptic culture. The suitability of beech sawdust as a model substrate in aseptic culture was assessed.
Department/SchoolTasmanian Institute of Agriculture (TIA)
PublisherInt Science Services/Balaban Publishers
Place of publicationRehovog Israel