University of Tasmania
Browse

File(s) not publicly available

Determination of Dietary Fiber and Carbohydrate Composition of Foods by a Modified Version of the Englyst Method

journal contribution
posted on 2023-05-16, 10:04 authored by Lichon, MJ, James, KW
A modified version of the Englyst procedure for determination of dietary fiber and carbohydrate fractions of foods is described. The method is based on hydrolysis of separated fractions and capillary gas chromatographic (GC) analysis of derivatized monomer sugars, UV photometry of uronic acids, gravimetric determination of residue, and analysis of nitrogen content of the residue by using a C, H, N elemental analyzer. Modifications of fractionation, derivatization, and GC procedures are discussed. Rigorous sample preparation is required for reproducibility of 200 mg test portions. Contami- nants interfering with chromatography must be minimized. Results for reference samples (wheat bran and Buerre Bosc pears) and white and wholemeal bread agree with literature values.

History

Publication title

Journal of AOAC International

Volume

79

Pagination

54-61

ISSN

1060-3271

Department/School

School of Natural Sciences

Publisher

Aoac International

Place of publication

Arlington, USA

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the chemical sciences

Usage metrics

    University Of Tasmania

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC