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Determination of the complete nucleotide sequences of RNA1 and RNA2 from greasy grouper (Epinephelus tauvina) nervous necrosis virus, Singapore strain
journal contribution
posted on 2023-05-16, 13:07 authored by Tan, C, Huang, B, Chang, SF, Ngoh, GH, Munday, BL, Chen, SC, Kwang, JThe complete nucleotide sequences of RNA1 and RNA2 from greasy grouper (Epinephelus tauvina) nervous necrosis virus (GGNNV), Singapore strain, were determined. 5′RACE and RNA ligation were used to obtain the complete nucleotide sequences of the 5′ and 3′ non-coding regions (NCRs). GGNNV RNA1 was determined to be 3103 nt long, containing an ORF of 982 aa, while GGNNV RNA2 was determined to be 1433 nt long, containing an ORF of 338 aa. Both GGNNV RNAs are longer than those of other published betanodavirus sequences and the additional nucleotides were located within the NCRs. Analysis of GGNNV RNA2 revealed that it is closely related to redspotted grouper nervous necrosis virus and that both grouper viruses share the same neutralization epitope. Predicted domains for six RNA-dependent RNA polymerase motifs and two putative ORFs (proteins B1 and B2) were confirmed by sequence analysis of GGNNV RNA1.
History
Publication title
Journal of General VirologyVolume
82Pagination
647-653ISSN
0022-1317Department/School
School of Health SciencesPublisher
Society of General MicrobiologyPlace of publication
United KingdomRepository Status
- Restricted