University Of Tasmania

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Development of 15 microsatellite loci from mulloway, Argyrosomus japonicus (Pisces: Sciaenidae) using next generation sequencing and an assessment of their cross amplification in other sciaenids

journal contribution
posted on 2023-05-19, 19:06 authored by Thomas BarnesThomas Barnes, Izzo, C, Bertozzi, T, Saint, KM, Donnellan, S, Hammer, MP, Gillanders, BM

Next generation sequencing was used to develop 15 new polymorphic microsatellite markers for the commercially and recreationally important fish mulloway (Argyrosomus japonicus). Improved knowledge of the genetic structure of the species is necessary for fisheries and environmental management. In a population of mulloway, we found that genetic variation and heterozygosity were high in most of our loci (mean number of alleles per locus = 9.46; mean heterozygosity = 0.660). To test the usefulness of our new markers for genetic research on other sciaenids, we cross amplified samples from eight other related sciaenid species, including three that form important fisheries and others that have significant conservation issues. We found that most of our new primers pairs produced scorable profiles in each of the other important sciaenids.


Publication title

Conservation Genetics Resources








Institute for Marine and Antarctic Studies


Springer Netherlands

Place of publication


Rights statement

Copyright 2013 Springer Science+Business Media Dordrecht

Repository Status

  • Restricted

Socio-economic Objectives

Fisheries - recreational freshwater; Wild caught fin fish (excl. tuna); Assessment and management of terrestrial ecosystems