Recent progress in the development of various diamond-related materials (DRMs) has induced a strong interest in their use as a stationary phase in various separation techniques. DRMs meet many requirements for use as a stationary phase in chromatography, including excellent mechanical and chemical stability, high thermal stability, low chemical reactivity of the surface, and biocompatibility. The general physicochemical properties of diamond and the preparation of different types of DRMs are reviewed, and an overview is provided of current and possible future applications in solid-phase extraction and various separation technologies.