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Digestive protease characterization, localization and adaptation in blacklip abalone (Haliotis rubra Leach)

journal contribution
posted on 2023-05-16, 13:06 authored by Edwards, SJ, Condon, CF
Digestive protease in blacklip abalone (Haliotis rubra Leach) from whole gut extracts was found to have dual pH optima at pH 3 and 10 and an optimum temperature of 45°C. Over the biologically relevant range of pH (5-8), protease activity dropped to a minimum at pH 5 (53% of the maximal rate at pH 3) rising gradually and continuously up to (and beyond) pH 8. Over the biologically relevant range of temperatures (9-24°C), protease activity increased continuously with activity at 24°C being 75% higher than activity at 9°C. Protease digestion was relatively uniform in gut extracts from sections containing gut contents. Digestion thus appears to be significantly extracellular (in the lumen of the gut). Analysis of gut sections washed free of food matter suggests the anatomical origin of protease from activity of gut regions in the order: digestive gland » salivary gland ≈ stomach > crop > intestine > upper oesophagus. Whole gut protease levels did not alter in response to a high protein artificial diet.

History

Publication title

Aquaculture Research

Volume

32

Pagination

95-102

ISSN

1355-557X

Department/School

School of Health Sciences

Publisher

Blackwell Science Ltd

Place of publication

Oxford, England

Repository Status

  • Restricted

Socio-economic Objectives

Fisheries - aquaculture not elsewhere classified

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