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Direct electrospinning of 3D auricle-shaped scaffolds for tissue engineering applications

journal contribution
posted on 2023-05-20, 05:13 authored by Walser, J, Kathryn Stok, Caversaccio, MD, Ferguson, SJ
Thirty-two poly(ε)caprolactone (PCL) scaffolds have been produced by electrospinning directly into an auricle-shaped mould and seeded with articular chondrocytes harvested from bovine ankle joints. After seeding, the auricle shaped constructs were cultured in vitro and analysed at days 1, 7, 14 and 21 for regional differences in total DNA, glycosaminoglycan (GAG) and collagen (COL) content as well as the expression of aggrecan (AGG), collagen type I and type II (COL1/2) and matrix metalloproteinase 3 and 13 (MMP3/13). Stress-relaxation indentation testing was performed to investigate regional mechanical properties of the electrospun constructs. Electrospinning into a conductive mould yielded stable 3D constructs both initially and for the whole in vitro culture period, with an equilibrium modulus in the MPa range. Rapid cell proliferation and COL accumulation was observed until week 3. Quantitative real time PCR analysis showed an initial increase in AGG, no change in COL2, a persistent increase in COL1, and only a slight decrease initially for MMP3. Electrospinning of fibrous scaffolds directly into an auricle-shape represents a promising option for auricular tissue engineering, as it can reduce the steps needed to achieve an implantable structure.

History

Publication title

Biofabrication

Volume

8

Pagination

25007

ISSN

1758-5082

Department/School

Menzies Institute for Medical Research

Publisher

Institute of Physics Publishing Ltd.

Place of publication

United Kingdom

Rights statement

©2016 IOP Publishing Ltd

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in engineering

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