University Of Tasmania

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Direct production of microstructured surfaces for planar chromatography using 3D printing

journal contribution
posted on 2023-05-19, 02:29 authored by Macdonald, NP, Currivan, SA, Tedone, L, Brett PaullBrett Paull
Through optimization of the printing process and orientation, a suitably developed surface area has been realized upon a 3D printed polymer substrate to facilitate chromatographic separations in a planar configuration. Using an Objet Eden 260VS 3D printer, polymer thin layer chromatography platforms were directly fabricated without any additional surface functionalization and successfully applied to the separation of various dye and protein mixtures. The print material was characterized using gas chromatography coupled to mass spectrometry and spectroscopic techniques such as infrared and Raman. Preliminary studies included the separation of colored dyes, whereby the separation performance could be visualized optically. Subsequent separations were achieved using fluorescent dyes and fluorescently tagged proteins. The separation of proteins was affected by differences in the isoelectric point (pI) and the ion exchange properties of the printed substrate. The simple chromatographic separations are the first achieved using an unmodified 3D printed stationary phase.


Publication title

Analytical Chemistry








School of Natural Sciences


Amer Chemical Soc

Place of publication

1155 16Th St, Nw, Washington, USA, Dc, 20036

Rights statement

Copyright 2017 American Chemical Society

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  • Restricted

Socio-economic Objectives

Expanding knowledge in the chemical sciences

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