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Dissecting blue light signal transduction pathway in leaf epidermis using a pharmacological approach
journal contributionposted on 2023-05-18, 10:23 authored by Branka Zivanovic, Svetlana ShabalaSvetlana Shabala, Elzenga, TJM, Sergey ShabalaSergey Shabala
Blue light signalling pathway in epidermal tissue isolated from the abaxial side of fully developed Vicia faba leaves was dissected by measuring the effect of inhibitors of second messengers on net K+, Ca2+ and H+ fluxes using non-invasive ion-selective microelectrodes (the MIFE system). Switching the blue light on-off caused transient changes of the ion fluxes. The effects of seven groups of inhibitors were tested in this study: CaM antagonists, ATPase inhibitors, Ca2+ anatagonists or chelators, agents affecting IP3 formation, redox system inhibitors, inhibitors of endomembrane Ca2+ transport systems and an inhibitor of plasma membrane Ca2+-permeable channels. Most of the inhibitors had a significant effect on steady-state (basal) net fluxes, as well as on the magnitude of the transient ion flux responses to blue light fluctuations. The data presented in this study suggest that redox signalling and, specifically, plasma membrane NADPH oxidase and coupled Ca2+ and K+ fluxes play an essential role in blue light signal transduction.
Department/SchoolTasmanian Institute of Agriculture (TIA)
Place of publication175 Fifth Ave, New York, USA, Ny, 10010
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