Distribution of Cardicola forsteri eggs in the gills of southern bluefin tuna (Thunnus maccoyii) (Castelnau, 1872)
journal contribution
posted on 2023-05-17, 11:44authored byNorte dos Santos, CD, Melanie Leef, Jones, J, Bott, N, Giblot-Ducray, D, Barbara NowakBarbara Nowak
The distribution of Cardicola forsteri eggs in the gills of southern bluefin tuna (Thunnus maccoyii) was analysed. Eggs were confirmed to be C. forsteri using laser-capture microdissection and quantitative polymerase chain reaction (qPCR) analyses. Prevalence of infection was significantly higher in the second gill arch (χ2 = 6.49, P < 0.05) than in the fourth gill arch, and significantly lower in the basal region of the second gill arch (χ2 = 6.29, d.f. = 2, P < 0.05). The intensity of infection was not significantly different between the gill arches (F = 0.03, d.f. = 1, P > 0.05). Similarly, there was no significant difference in the intensity of C. forsteri eggs between the gill arches (F = 3.43, d.f. = 2, P > 0.05), or at different depth of sectioning (F = 0.08, d.f. = 1.12, P > 0.05). Results suggest that the presence of C. forsteri eggs in the gills of tuna is more likely to be detected by sampling the second gill arch. Furthermore, targeting the middle region and increasing the sectioning depth may reduce the proportion of false negatives.
History
Publication title
Aquaculture: An International Journal Devoted to Fundamental Aquatic Food Resources
Volume
344-349
Pagination
54-57
ISSN
0044-8486
Department/School
Institute for Marine and Antarctic Studies
Publisher
Elsevier Science Bv
Place of publication
Po Box 211, Amsterdam, Netherlands, 1000 Ae
Rights statement
Copyright 2012 Elsevier B.V.
Repository Status
Restricted
Socio-economic Objectives
Aquaculture crustaceans (excl. rock lobster and prawns)