116003 - Early CCR6 expression on B cells - Final author version.pdf (182.56 kB)
Early CCR6 expression on B cells modulates germinal centre kinetics and efficient antibody responses
journal contributionposted on 2023-05-19, 04:00 authored by Reimer, D, Adrian Lee, Bannan, J, Fromm, P, Kara, EE, Comerford, I, McColl, S, Wiede, F, Mielenz, D, Heinrich KornerHeinrich Korner
The CC-chemokine receptor 6 (CCR6) can be detected on naive and activated B cells. Counterintuitively, its absence accelerates the appearance of germinal centres (GCs) and increases the production of low-affinity antibodies. The detailed mechanism of CCR6 function during the humoral response has remained elusive, but previously we identified a distinct CCR6high B-cell population in vivo early after antigenic challenge. In this study, we defined this population specifically as early, activated pre-GC B cells. In accordance, we show that CCR6 is upregulated rapidly within hours on the protein or mRNA level after activation in vitro. In addition, only activated B cells migrated specifically towards CCL20, the specific ligand for CCR6. Lack of CCR6 increased the dark zone/light zone ratio of GC and led to decreased antigen-specific IgG1 and IgG2a antibody generation in a B-cell intrinsic manner in mixed bone marrow chimeras. In contrast, antigen-specific IgM responses were normal. Hence, CCR6 negatively regulates entry of activated, antigen-specific pre-GC B cells into the GC reaction.
Publication titleImmunology and Cell Biology
Department/SchoolMenzies Institute for Medical Research
PublisherNature Publishing Group
Place of publicationUnited Kingdom
Rights statementCopyright 2016 Australasian Society for Immunology Inc.