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Effect of cloned inhibitor resistant TEM B-lactamases on the susceptibility of H. influenzae to amoxicillin/clavulanate
journal contributionposted on 2023-05-16, 21:01 authored by Stephen TristramStephen Tristram, Burdach, JG
Objectives: To determine the effect of cloned inhibitor-resistant TEM Î²-lactamases (IRTs) on the susceptibility of Haemophilus influenzae to amoxicillin/clavulanate. Methods: IRT-2, -4 and -5 genes with various promoters were cloned into control strains of H. influenzae and the amoxicillin/clavulanate MICs were measured using Etests. Results: IRT enzymes were able to raise the amoxicillin/clavulanate MICs to between 0.38/0.19 and 4.0/2.0 mg/L depending on the IRT and promoter genotype, compared with MICs of 0.19/0.09 to 0.5/0.25 mg/L for the corresponding strains with TEM-1. Strains with an IRT and altered penicillin-binding proteins had amoxicillin/clavulanate MICs as high as 8.0/4.0 mg/L. Conclusions: Cloned IRT enzymes in H. influenzae raise the amoxicillin/ clavulanate MICs to an extent comparable to naturally occurring strains with decreased amoxicillin/clavulanate susceptibility. Â© The Author 2007. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.
Publication titleJournal of Antimicrobial Chemotherapy
Department/SchoolSchool of Health Sciences
PublisherOxford University Press
Place of publicationOxford, UK