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Effects of exogenously-applied L-ascorbic acid on root expansive growth and viability of the border-like cells
journal contribution
posted on 2023-05-19, 23:42 authored by Li, X, Makavitskaya, M, Samokhina, V, Mackievic, V, Navaselsky, I, Hryvusevich, P, Smolikova, G, Medvedev, S, Sergey ShabalaSergey Shabala, Yu, M, Demidchik, VFunctions of exogenous L-ascorbic acid in plant roots are poorly understood. Recent study by Makavitskaya et al. (doi.org/10.1093/jxb/ery056) has demonstrated that exogenous ascorbate can be released from roots in response to salt stress, and can trigger elevation in the cytosolic free Ca2+. Here, we report that exogenous ascorbate significantly modifies root elongation in Arabidopsis thaliana. Using a medium exchange technique, we have shown that 10-100 µM ascorbate induces small but significant increase in root elongation while higher levels cause its dramatic decrease. Root border cells of Pisum sativum have been losing viability twice faster in the presence of ascorbate that under control conditions, as tested by the confocal microscopy and a combined staining with propidium iodide and fluorescein diacetate.
History
Publication title
Plant Signaling & BehaviorVolume
13Issue
9Pagination
e1514895ISSN
1559-2316Department/School
Tasmanian Institute of Agriculture (TIA)Publisher
Taylor & Francis IncPlace of publication
United StatesRights statement
Copyright 2018 Taylor & Francis Group, LLCRepository Status
- Restricted