Effects of iron limitation on growth and carbon metabolism in oceanic and coastal heterotrophic bacteria
journal contribution
posted on 2023-05-18, 01:00authored byFourquez, M, Devez, A, Schaumann, A, Gueneugues, A, Jouenne, T, Obernosterer, I, Blain, S
We investigated the metabolic response to iron (Fe) limitation of two bacterial strains of Alteromonas macleodii, isolated from a coastal and an oceanic marine environment. Bacteria were grown under Fe-limited and Fe-replete conditions, and comparative analyses of cellular properties and total proteomes were conducted. Respiration was reduced by a factor of two in both strains, but the growth rate of the oceanic strain was less affected by Fe limitation (reduced by 1.2-fold) than the coastal strain (reduced by 2-fold). Fe limitation led to significant changes in the expression of several key enzymes associated with carbon catabolism, specifically those involved in the citric acid cycle, glycolysis, and oxidative phosphorylation. The strain-specific overall responses to Fe limitation were in part reflected in different metabolic strategies of the carbon metabolism and energy acquisition. Our study provides novel insights on how Fe limitation can affect bacterial heterotrophic metabolism, and how this could influence the coupling of the Fe and carbon cycles in the ocean.
History
Publication title
Limnology and Oceanography
Volume
59
Pagination
349-360
ISSN
0024-3590
Department/School
Institute for Marine and Antarctic Studies
Publisher
Amer Soc Limnology Oceanography
Place of publication
5400 Bosque Blvd, Ste 680, Waco, USA, Tx, 76710-4446
Rights statement
Copyright 2014 by the Association for the Sciences of Limnology and Oceanography Inc.
Repository Status
Restricted
Socio-economic Objectives
Oceanic processes (excl. in the Antarctic and Southern Ocean)