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Electroosmotic flow assisted pseudophase to pseudophase microextraction for stacking in capillary zone electrophoresis

journal contribution
posted on 2023-05-21, 10:45 authored by Andaravaas Patabadige Jude VaasAndaravaas Patabadige Jude Vaas, Joselito Quirino
A stacking technique is proposed to improve the poor detection sensitivity of capillary zone electrophoresis (CZE) with UV detection. A long injection (e.g., 12.4 cm plug) of model anionic analytes prepared in a dilute solution of hexadecyltrimethylammonium bromide (CTAB) was enriched 26-34-x (compared to a typical or 2.1 mm sample injection) via the injection of a micellar solution of sodium dodecyl sulfate (SDS) prior to CZE separation. During sample injection, the CTAB formed a stationary pseudophase coating, which trapped the analytes at the inner walls of a fused silica capillary. The SDS micelles then released the CTAB admicelles via the formation of solution CTAB-SDS catanionic micelles during SDS plug injection and voltage application. As the SDS micelles moved through the sample zone, the formation of the catanionic micelles then released and accumulated the analytes at the front of the injected SDS zone. The stacking technique is called electroosmotic flow (EOF) assisted pseudophase to pseudophase microextraction because the EOF was essential for the formation of CTAB-SDS catanionic micelles for microextraction. Also, the CTAB and SDS aggregates are both pseudophases, which were used to retain and release the analytes from the capillary wall, respectively.

History

Publication title

Journal of Chromatography A

Volume

1660

Article number

462654

Number

462654

Pagination

1-5

ISSN

0021-9673

Department/School

School of Natural Sciences

Publisher

Elsevier Science Bv

Place of publication

Po Box 211, Amsterdam, Netherlands, 1000 Ae

Rights statement

Crown Copyright © 2021 Published by Elsevier B.V. All rights reserved.

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the chemical sciences

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