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Elution, detection, and quantification of polio I, bacteriophages, Salmonella Montevideo, and Escherichia coli O157:H7 from seeded strawberries and tomatoes

journal contribution
posted on 2023-05-19, 02:54 authored by Lukasik, J, Bradley, ML, Scott, TM, Hsu, W-Y, Farrah, SR, Mark TamplinMark Tamplin
This study compared the effect of different physical and chemical treatments of strawberries and tomatoes to determine their ability to recover seeded viral and bacterial pathogens from produce surfaces. Solutions of salts, amino acids, complex media, and detergents were compared as eluants. Phosphate-buffered saline (PBS) containing 0.1% Tween 80 eluted the highest number of seeded microorganisms. Elution with this defined solution was then compared under different conditions of physical agitation. Rotary shaking for 20 min at 36°C eluted higher numbers of viruses and bacteria than did low- or high-speed stomaching. Commercially available and laboratory prepared bacteriological differential media were compared for their ability to recover and distinguish eluted Salmonella Montevideo and Escherichia coli O157:H7 strains from seeded produce. The recovery of seeded bacterial pathogens was low when differential media containing selective ingredients were used (Mac-Conkey sorbitol agar, XLD agar, MacConkey agar). Highest recoveries were obtained on a medium consisting of tryptic soy agar supplemented with sodium thiosulfate and ferric ammonium citrate compared with selective media that inhibited up to 50% of the growth of the eluted microorganisms.

History

Publication title

Journal of Food Protection

Volume

64

Pagination

292-297

ISSN

0362-028X

Department/School

Tasmanian Institute of Agriculture (TIA)

Publisher

Int Assoc Food Protection

Place of publication

6200 Aurora Ave Suite 200W, Des Moines, USA, Ia, 50322-2863

Rights statement

Copyright 2001 International Association for Food Protection

Repository Status

  • Restricted

Socio-economic Objectives

Food safety

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