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Gaikwad et al_00487-2022.pdf (1.45 MB)

Endothelial-to-mesenchymal transition: a precursor to pulmonary arterial remodelling in patients with idiopathic pulmonary fibrosis

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posted on 2023-07-31, 01:52 authored by Archana GaikwadArchana Gaikwad, Wenying LuWenying Lu, Surajit Dey, Prem BhattaraiPrem Bhattarai, Greg Haug, Josie Larby, Collin Chia, Jade Jaffar, Glen Westall, Gurpreet Kaur Singhera, Tillie-Louise Hackett, Mathew Eapen, Sukhwinder SohalSukhwinder Sohal
BACKGROUND: We have previously reported arterial remodelling in patients with idiopathic pulmonary fibrosis (IPF) and suggested that endothelial-to-mesenchymal transition (EndMT) might be central to these changes. This study aims to provide evidence for active EndMT in IPF patients. METHODS: Lung resections from 13 patients with IPF and 15 normal controls (NCs) were immunostained for EndMT biomarkers: vascular endothelial cadherin (VE-cadherin), neural cadherin (N-cadherin), S100A4 and vimentin. Pulmonary arteries were analysed for EndMT markers by using computer- and microscope-assisted image analysis software Image ProPlus7.0. All the analysis was done with observer blinded to subject and diagnosis. RESULTS: Increased expression of mesenchymal markers N-cadherin (p<0.0001), vimentin (p<0.0001) and S100A4 (p<0.05) was noted with downregulation of junctional endothelial VE-cadherin (p<0.01) in the intimal layer of the arteries from patients with IPF compared to NCs. Cadherin switch was observed in IPF patients, showing increase in endothelial N-cadherin and decrease in VE-cadherin (p<0.01). There was also VE-cadherin shift from junctions to cytoplasm (p<0.01), effecting endothelial cell integrity in patients with IPF. In IPF, individual mesenchymal markers vimentin and N-cadherin negatively correlated with diffusing capacity of the lungs for carbon monoxide (r'= -0.63, p=0.03 and r'= -0.66, p=0.01). Further, N-cadherin positively correlated with arterial thickness (r'=0.58, p=0.03). CONCLUSION: This is the first study to demonstrate active EndMT in size-based classified pulmonary arteries from IPF patients and potential role in driving remodelling changes. The mesenchymal markers had a negative impact on the diffusing capacity of the lungs for carbon monoxide. This work also informs early origins of pulmonary hypertension in patients with IPF.

History

Sub-type

  • Article

Publication title

ERJ OPEN RESEARCH

Medium

Electronic-eCollection

Volume

9

Issue

2

Pagination

16

eISSN

2312-0541

ISSN

2312-0541

Department/School

Health Sciences

Publisher

EUROPEAN RESPIRATORY SOC JOURNALS LTD

Publication status

  • Published

Place of publication

England

Event Venue

Respiratory Translational Research Group, Department of Laboratory Medicine, School of Health Sciences, College of Health and Medicine, University of Tasmania, Launceston, TAS, Australia.

Rights statement

Copyright ©The authors 2023 This version is distributed under the terms of the Creative Commons Attribution Non- Commercial Licence 4.0.

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