Endothelial Na+ -D-glucose cotransporter: No Role in Insulin-mediated Glucose Uptake
journal contribution
posted on 2023-05-16, 17:20 authored by Kolka, CM, Stephen RattiganStephen Rattigan, Stephen RichardsStephen Richards, Barrett EJ, EJ, Michael ClarkMichael ClarkA recent report indicates that the Na+-D-glucose cotransporter SGLT1 is present in capillaries of skeletal muscle and is required for insulin-mediated glucose uptake in myocytes. This result is based on the complete inhibition of insulin-mediated muscle glucose uptake by phlorizin, an inhibitor of SGLT1. Using the pump-perfused rat hind limb, we measured glucose uptake, lactate efflux, and radioactive 2-deoxyglucose uptake into individual muscles with saline (control), phlorizin, insulin, and insulin plus phlorizin, as well as with saline and insulin using normal and low Na+ perfusion buffer. Insulin-mediated glucose uptake was not inhibited after correction for phlorizin interference in the glucose assay. Lactate efflux and 2-deoxyglucose uptake by individual muscles were unaffected by phlorizin. Low Na+ buffer did not affect insulin-mediated glucose uptake, lactate efflux, or 2-deoxyglucose uptake. We conclude that endothelial SGLT1 exerts no barrier for glucose delivery to myocytes. © Georg Thieme Verlag KG Stuttgart.
Funding
National Institutes of Health
History
Publication title
Hormone and Metabolic ResearchVolume
37Issue
11Pagination
657-661ISSN
0018-5043Department/School
Tasmanian School of MedicinePublisher
Georg Thieme VerlagPlace of publication
StuttgartRepository Status
- Restricted
Socio-economic Objectives
Clinical health not elsewhere classifiedUsage metrics
Categories
Keywords
Licence
Exports
RefWorksRefWorks
BibTeXBibTeX
Ref. managerRef. manager
EndnoteEndnote
DataCiteDataCite
NLMNLM
DCDC