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Enhanced osteogenic differentiation of human fetal cartilage rudiment cells on graphene oxide-PLGA hybrid microparticles

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posted on 2023-05-20, 06:26 authored by Stuart ThickettStuart Thickett, Hamilton, E, Yogeswaran, G, Zetterlund, PB, Farrugia, BL, Lord, MS
Poly(d,l–lactide–co–glycolide) (PLGA) has been extensively explored for bone regeneration applications; however, its clinical use is limited by low osteointegration. Therefore, approaches that incorporate osteoconductive molecules are of great interest. Graphene oxide (GO) is gaining popularity for biomedical applications due to its ability to bind biological molecules and present them for enhanced bioactivity. This study reports the preparation of PLGA microparticles via Pickering emulsification using GO as the sole surfactant, which resulted in hybrid microparticles in the size range of 1.1 to 2.4 µm based on the ratio of GO to PLGA in the reaction. Furthermore, this study demonstrated that the hybrid GO-PLGA microparticles were not cytotoxic to either primary human fetal cartilage rudiment cells or the human osteoblast-like cell line, Saos-2. Additionally, the GO-PLGA microparticles promoted the osteogenic differentiation of the human fetal cartilage rudiment cells in the absence of exogenous growth factors to a greater extent than PLGA alone. These findings demonstrate that GO-PLGA microparticles are cytocompatible, osteoinductive and have potential as substrates for bone tissue engineering.

History

Publication title

Journal of Functional Biomaterials

Volume

10

Article number

33

Number

33

Pagination

1-12

ISSN

2079-4983

Department/School

School of Natural Sciences

Publisher

MDPI AG

Place of publication

Basel, Switzerland

Rights statement

Copyright 2019 The Authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

Repository Status

  • Open

Socio-economic Objectives

Expanding knowledge in the chemical sciences; Expanding knowledge in engineering

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