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Enhancement of neurite outgrowth in neuron cancer stem cells on 3-D collagen scaffolds

journal contribution
posted on 2023-05-19, 10:49 authored by Chen, CH, Kuo, SM, Guei-Sheung LiuGuei-Sheung Liu, Chen, WNU, Chuang, CW, Liu, LF
Collagen is one component of the extracellular matrix that has been widely used for constructive remodeling to facilitate cell growth and differentiation. The 3-D distribution and growth of cells within the porous scaffold suggest a clinical significance for nerve tissue engineering. In the current study, we investigated proliferation and differentiation of neuron cancer stem cells (NCSCs) on a 3-D porous collagen scaffold that mimics the natural extracellular matrix. We first generated green fluorescence protein (GFP) expressing NCSCs using a lentiviral system to instantly monitor the transitions of morphological changes during growth on the 3-D scaffold. We found that proliferation of GFP-NCSCs increased, and a single cell mass rapidly grew with unrestricted expansion between days 3 and 9 in culture. Moreover, immunostaining with neuronal nuclei (NeuN) revealed that NCSCs grown on the 3-D collagen scaffold significantly enhanced neurite outgrowth. Our findings confirmed that the 80 μm porous collagen scaffold could enhance attachment, viability and differentiation of the cancer neural stem cells. This result could provide a new application for nerve tissue engineering and nerve regeneration.


Publication title

Biochemical and Biophysical Research Communications








Menzies Institute for Medical Research


United States

Place of publication

Elsevier Inc.

Rights statement

Copyright 2012 Elsevier Inc

Repository Status

  • Restricted

Socio-economic Objectives

Clinical health not elsewhere classified

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