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Evaluation of quantitative polymerase chain reaction to assess nosZ gene prevalence in mixed microbial communities

journal contribution
posted on 2023-05-16, 20:59 authored by Sicilliano, SD, Wai, M, Shane PowellShane Powell
The usefulness of quantitative polymerase chain reaction (QPCR) to measure nosZ gene prevalence in a multitemplate reaction was assessed by comparing 19 nosZ template DNA samples and 91 model communities. Efficiencies of the QPCR varied but were not significantly different among nosZ genotypes and were not linked to genetic distance from Ralstonia eutropha. nosZ genotype QPCR efficiencies obtained from isolated denitrifiers were higher (84.8%) than those obtained from excised denaturing gradient gel electrophoresis bands or clones of PCR products from total community DNA (ca. 60%). Analysis of the model communities indicated that QPCR accurately predicts gene prevalence in communities composed of up to six templates. © 2007 NRC.

History

Publication title

Canadian Journal of Microbiology

Volume

53

Issue

5

Pagination

636-642

ISSN

0008-4166

Department/School

Tasmanian Institute of Agriculture (TIA)

Publisher

NRC Research Press

Place of publication

Canada

Repository Status

  • Restricted

Socio-economic Objectives

Assessment and management of terrestrial ecosystems

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