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Expression of a single gene encoding microbody NAD-malate dehydrogenase during glyoxysome and peroxisome development in cucumber

journal contribution
posted on 2023-05-18, 11:29 authored by Kim, D-J, Steven SmithSteven Smith
A full-length cDNA clone encoding microbody NAD +-dependent malate dehydrogenase (MDH) of cucumber has been isolated. The deduced amino acid sequence is 97 ~o identical to glyoxysomal MDH (gMDH) of watermelon, including the amino terminal putative transit peptide. The cucumber genome contains only a single copy of this gene. Expression of this mdh gene increases dramatically in cotyledons during the few days immediately following seed imbibition, in parallel with genes encoding isocitrate lyase (ICL) and malate synthase (MS), two glyoxylate cycle enzymes. The level of MDH, ICL and MS mRNAs then declines, but then MDH mRNA increases again together with that of peroxisomal NAD + - dependent hydroxypyruvate reductase (HPR). The mdh gene is also expressed during cotyledon senescence, together with hpr, icl and ms genes. These results indicate that a single gene encodes MDH which functions in both glyoxysomes and peroxisomes. In contrast to icI and ms genes, expression of the mdh gene is not activated by incubating detached green cotyledons in the dark, nor is it affected by exogenous sucrose in the incubation medium. The function of this microbody MDH and the regulation of its synthesis are discussed.

History

Publication title

Plant Molecular Biology

Volume

26

Issue

6

Pagination

1833-1841

ISSN

0167-4412

Department/School

School of Natural Sciences

Publisher

Kluwer Academic Publ

Place of publication

Van Godewijckstraat 30, Dordrecht, Netherlands, 3311 Gz

Rights statement

Copyright 1994 Kluwer Academic Publishers

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the biological sciences

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