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Expression of chloramphenicol resistance specified by plasmid pHY416 hosted in Corynebacterium glutamicum
journal contributionposted on 2023-05-17, 08:19 authored by Margaret BritzMargaret Britz, Best, GR
Chloramphenicol (Cm)-resistant colonies of Corynebacterium glutamicum strain AS019 hosting plasmid pHY416 occurred at a frequency of 4Ã—10-7 when this strain, which normally expresses kanamycin (Km) resistance only, was placed under positive selection. These isolates produced a chloramphenicol acetyltransferase (CAT) and were tenfold more resistant to Cm than the parent strain. Resistance was lost in the absence of Cm selection but could be reselected from Km-resistant clones by reapplying Cm selection. Restriction endonuclease analyses of plasmids extracted from Cm-sensitive and-resistant strains indicated that expression of CAT activity corresponded to a loss of 0.9 kb of DNA from plasmid pHY416. Â© 1986 Springer-Verlag New York Inc.
Publication titleCurrent Microbiology: An International Journal
Department/SchoolCollege Office - College of Sciences and Engineering
Place of publication175 Fifth Ave, New York, USA, Ny, 10010