Fabrication and characterisation of capillary polymeric monoliths incorporating continuous stationary phase gradients
journal contribution
posted on 2023-05-17, 07:14 authored by Currivan, S, Connolly, D, Gillespie, E, Brett PaullBrett PaullPolymeric monoliths in capillary formats have been fabricated incorporating a gradient of charged functional groups along their length. Scanning capacitively coupled contactless conductivity detection (sC4D) was then used to measure the conductive response of the stationary phase and characterise the relative axial distribution of functional groups along the column length. Gradients of 2-acrylamido-2-methyl-1-propanesulphonic acid were prepared using either photografting methods or by filling a capillary column with segmented plugs of monomer mixtures each containing incrementally higher concentrations of the functional monomer. The utility of sC4D as a rapid and non-invasive tool for assessing the slope of a variety of gradient configurations is demonstrated. Repeatability of the sC4D measurements was <1.7% RSD. Columns with a gradient of covalently bonded iminodiacetic acid were also produced. Changes in the gradient slope were observed after chelation of copper on the stationary phase via a reduction of the conductive response. The effect upon the observed gradient profile of changing the comonomer composition during column fabrication was studied. © 2010 WILEY-VCH Verlag GmBH & Co. KGaA.
History
Publication title
Journal of Separation ScienceVolume
33Issue
4-5Pagination
484-492ISSN
1615-9306Department/School
School of Natural SciencesPublisher
Wiley-V C H Verlag GmbhPlace of publication
GermanyRepository Status
- Restricted
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