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Fish gill damage by harmful microalgae newly explored by microelectrode ion flux estimation techniques

journal contribution
posted on 2023-05-19, 21:36 authored by Mardones, JI, Svetlana ShabalaSvetlana Shabala, Sergey ShabalaSergey Shabala, Dorantes-Aranda, JJ, Andreas SegerAndreas Seger, Gustaaf HallegraeffGustaaf Hallegraeff

Harmful algal blooms (HAB) are responsible for massive mortalities of wild and aquacultured fish due to noticeable gill damage, but the precise fish-killing mechanisms remain poorly understood. A non-invasive microelectrode ion flux estimation (MIFE) technique was successfully applied to assess changes in membrane-transport processes in a model fish gill cell line exposed to harmful microplankton. Net Ca2+, H+, K+ ion fluxes in the rainbow trout cell line RTgill-W1 were monitored before and after addition of lysed cells of this Paralytic Shellfish Toxins (PST) producer along with purified endocellular dinoflagellate PST. It was demonstrated that PST alone do not play a role in fish gill damage during A. catenella outbreaks as previously thought, but that other ichthyotoxic metabolites from lysed algal cells (i.e. lipid peroxidation products or other unknown metabolites) result in net K+ efflux from fish gill cells and thereby gill cell death.

Funding

Australian Research Council

History

Publication title

Harmful Algae

Volume

80

Pagination

55-63

ISSN

1568-9883

Department/School

Institute for Marine and Antarctic Studies

Publisher

Elsevier BV

Place of publication

Netherlands

Rights statement

Copyright 2018 Elsevier B.V.

Repository Status

  • Restricted

Socio-economic Objectives

Fisheries - aquaculture not elsewhere classified

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