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Fractal kinetic behavior of plasmin on the surface of fibrin meshwork

journal contribution
posted on 2023-05-19, 04:15 authored by Varju, I, Kiril Tenekedjiev, Keresztes, Z, Pap, AE, Szabo, L, Thelwell, C, Longstaff, C, Machovich, R, Kolev, K
Intravascular fibrin clots are resolved by plasmin acting at the interface of gel-phase substrate and fluid-borne enzyme. The classic Michaelis–Menten kinetic scheme cannot describe satisfactorily this heterogeneous-phase proteolysis because it assumes homogeneous well-mixed conditions. A more suitable model for these spatial constraints, known as fractal kinetics, includes a time-dependence of the Michaelis coefficient KmF = Km0F(1 + t)h, where h is a fractal exponent of time, t. The aim of the present study was to build up and experimentally validate a mathematical model for surface-acting plasmin that can contribute to a better understanding of the factors that influence fibrinolytic rates. The kinetic model was fitted to turbidimetric data for fibrinolysis under various conditions. The model predicted Km0F= 1.98 μM and h = 0.25 for fibrin composed of thin fibers and Km0F= 5.01 μM and h = 0.16 for thick fibers in line with a slower macroscale lytic rate (due to a stronger clustering trend reflected in the h value) despite faster cleavage of individual thin fibers (seen as lower Km0F). ε-Aminocaproic acid at 1 mM or 8 U/mL carboxypeptidase-B eliminated the time-dependence of KmF and increased the lysis rate suggesting a role of C-terminal lysines in the progressive clustering of plasmin. This fractal kinetic concept gained structural support from imaging techniques. Atomic force microscopy revealed significant changes in plasmin distribution on a patterned fibrinogen surface in line with the time-dependent clustering of fluorescent plasminogen in confocal laser microscopy. These data from complementary approaches support a mechanism for loss of plasmin activity resulting from C-terminal lysine-dependent redistribution of enzyme molecules on the fibrin surface.

History

Publication title

Biochemistry

Volume

53

Issue

40

Pagination

6348-6356

ISSN

0006-2960

Department/School

Australian Maritime College

Publisher

American Chemical Society

Place of publication

USA

Rights statement

© 2014 American Chemical Society

Repository Status

  • Restricted

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