Function of NHX-type transporters in improving rice tolerance to aluminum stress and soil acidity

Plant sodium (potassium)/proton (Na⁺(K⁺)/H⁺ antiporters of the NHX family have been extensively characterized as they are related to the enhancement of salt tolerance. However, no previous study has reported NHX transporter functions in plant tolerance to Al³⁺ toxicity. In this study, we demonstrate their role as a component of the Al³⁺ stress tolerance mechanism. We show that the ectopic expression of either HtNHX1 or HtNHX2 , from Helianthus tuberosus plant, in rice (located at vacuole and endosome, respectively) could also enhance rice tolerance to Al³⁺ stress and soil acidity. Expression of either HtNHX1 or HtNHX2 reduced the inhibitory effect of Al³⁺ on the rice root elongation rate; both genes were reported to be equally effective in improvement of stress conditions. Expression of HtNHX1 enhanced Al³⁺-trigged-secretion of citrate acids, rhizosphere acidification, and also reduced K⁺ efflux from root tissues. In contrast, expression of HtNHX2 prevented Al³⁺-trigged-decrease of H⁺ influx into root tissues. Al³⁺-induced damage of the cell wall extensibility at the root tips was impaired by either HtNHX1 or HtNHX2. Co-expression of HtNHX1 and HtNHX2 further improved rice growth, particularly under the Al³⁺ stress conditions. The results demonstrate that HtNHX1 and HtNHX2 improved rice tolerance to Al³⁺ via different mechanisms by altering the K⁺ and H⁺ fluxes and the cell wall structure.