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Fungal identification using a Bayesian classifier and the Warcup training set of internal transcribed spacer sequences
journal contributionposted on 2023-05-19, 02:48 authored by Deshpande, V, Wang, Q, Greenfield, P, Michael CharlestonMichael Charleston, Porras-Alfaro, A, Kuske, CR, Cole, JR, Midgley, DJ, Tran-Dinh, N
Fungi are key organisms in many ecological processes and communities. Rapid and low cost surveys of the fungal members of a community can be undertaken by isolating and sequencing a taxonomically informative genomic region, such as the ITS (internal transcribed spacer), from DNA extracted from a metagenomic sample, and then classifying these sequences to determine which organisms are present. This paper announces the availability of the Warcup ITS training set and shows how it can be used with the Ribosomal Database Project (RDP) Bayesian Classifier to rapidly and accurately identify fungi using ITS sequences. The classifications can be down to species level and use conventional literature-based mycological nomenclature and taxonomic assignments.
Department/SchoolSchool of Natural Sciences
PublisherNew York Botanical Garden
Place of publicationPublications Dept, Bronx, USA, Ny, 10458
Rights statementCopyright 2016 by The Mycological Society of America, Lawrence