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Further investigations of the effects of anti-β2GP1 antibodies on collagen-induced platelet aggregation

journal contribution
posted on 2023-05-19, 13:32 authored by Ho, YC, Kiran AhujaKiran Ahuja, Adams, MJ
Anti-beta-2-glycoprotein 1 (anti-β2GP1) antibodies are associated with increased thrombotic risk in patients with autoimmune disease. There is conflicting evidence on the effects of anti-β2GP1 antibodies on platelets, with both enhanced and inhibited aggregation previously reported. However, previous studies did not include isotype antibodies to ensure the observed effects were due to anti-β2GP1 antibodies. The aims of this study were to (1) investigate the effects of anti-β2GP1 antibodies on collagen-induced platelet aggregation in parallel with negative control (buffer normal saline) and isotype control antibodies and (2) determine the lupus anticoagulant (LA) activity of anti-β2GP1 antibodies used. Three animal-derived anti-human-β2GP1 antibodies (1.25, 2.5, and 5 μg/mL) incubated with healthy platelet-rich plasma were activated by collagen (2.5 μg/mL). Each anti-β2GP1 antibody demonstrated the inhibition of aggregation compared to negative control, but not to isotype control. No anti-β2GP1 antibody demonstrated LA activity, suggesting they were probably nonpathological. This study highlights both negative and isotype control markers are important to validate the effects of anti-β2GP1 antibodies. Assays to measure anti-domain I-β2GP1 antibodies are recommended to be used in conjunction with functional measures to further investigate the effects of anti-β2GP1 antibodies.


Publication title

Clinical and Applied Thrombosis - Hemostasis

Article number

Epub ahead of print


Epub ahead of print






School of Health Sciences


Westminster Publ Inc

Place of publication

New York

Rights statement

Copyright 2017 the Authors. Licensed under Creative Commons Attribution-NonCommercial 4.0 license (CC BY-NC)

Repository Status

  • Restricted

Socio-economic Objectives

Diagnosis of human diseases and conditions

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