University of Tasmania
149701 - Genetic analysis of early phenology in lentil.pdf (3.58 MB)

Genetic analysis of early phenology in lentil identifies distinct loci controlling component traits

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Modern-day domesticated lentil germplasm is generally considered to form three broad adaptation groups: Mediterranean, South Asian and northern temperate, which correspond to the major global production environments. Reproductive phenology plays a key role in lentil adaptation to this diverse ecogeographic variation. Here, we dissect the characteristic earliness of the pilosae ecotype, suited to the typically short cropping season of South Asian environments. We identified two loci, DTF6a and DTF6b, at which dominant alleles confer early flowering, and we show that DTF6a alone is sufficient to confer early flowering under extremely short photoperiods. Genomic synteny confirmed the presence of a conserved cluster of three florigen (FT) gene orthologs among potential candidate genes, and expression analysis in near-isogenic material showed that the early allele is associated with a strong derepression of the FTa1 gene in particular. Sequence analysis revealed a 7.4 kb deletion in the FTa1-FTa2 intergenic region in the pilosae parent, and a wide survey of over 350 accessions with diverse origin showed that the dtf6a allele is predominant in South Asian material. Collectively, these results contribute to understanding the molecular basis of global adaptation in lentil, and further emphasize the importance of this conserved genomic region for adaptation in temperate legumes generally.


Publication title

Journal of Experimental Botany










School of Natural Sciences


Oxford Univ Press

Place of publication

Great Clarendon St, Oxford, England, Ox2 6Dp

Rights statement

© The Author(s) 2022. This is a pre-copyedited, author-produced version of an article accepted for publication in Journal of Experimental Botany following peer review. The version of record, Vinodan Rajandran, Raul Ortega, Jacqueline K Vander Schoor, Jakob B Butler, Jules S Freeman, Valerie F G Hecht, Willie Erskine, Ian C Murfet, Kirstin E Bett, James L Weller, Genetic analysis of early phenology in lentil identifies distinct loci controlling component traits, Journal of Experimental Botany, 2022, erac107, is available online at:

Repository Status

  • Open

Socio-economic Objectives

Expanding knowledge in the biological sciences