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Genetic control of cuticular wax compounds in Eucalyptus globulus
journal contributionposted on 2023-05-18, 15:22 authored by Gosney, BJ, Bradley PottsBradley Potts, Julianne O'Reilly-WapstraJulianne O'Reilly-Wapstra, Rene VaillancourtRene Vaillancourt, Hugh FitzgeraldHugh Fitzgerald, Noel DaviesNoel Davies, Jules FreemanJules Freeman
- Plant cuticular wax compounds perform functions that are essential for the survival of terrestrial plants. Despite their importance, the genetic control of these compounds is poorly understood outside of model taxa. Here we investigate the genetic basis of variation in cuticular compounds in Eucalyptus globulus using quantitative genetic and quantitative trait loci (QTL) analyses.
- Quantitative genetic analysis was conducted using 246 open-pollinated progeny from 13 native sub-races throughout the geographic range. QTL analysis was conducted using 112 clonally replicated progeny from an outcross F2 population.
- Nine compounds exhibited significant genetic variation among sub-races with three exhibiting signals of diversifying selection. Fifty-two QTL were found with co-location of QTL for related compounds commonly observed. Notable among these was the QTL for five wax esters, which co-located with a gene from the KCS family, previously implicated in the biosynthesis of cuticular waxes in Arabidopsis.
- In combination, the QTL and quantitative genetic analyses suggest the variation and differentiation in cuticular wax compounds within E. globulus has a complex genetic origin. Sub-races exhibited independent latitudinal and longitudinal differentiation in cuticular wax compounds, likely reflecting processes such as historic gene flow and diversifying selection acting upon genes that have diverse functions in distinct biochemical pathways.
Australian Research Council
Publication titleNew Phytologist
Department/SchoolSchool of Natural Sciences
PublisherBlackwell Publishing Ltd
Place of publication9600 Garsington Rd, Oxford, England, Oxon, Ox4 2Dg
Rights statementCopyright? 2015 University of Tasmania. New Phytologist Copyright? 2015 New Phytologist Trust