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Genetic diversity and QTL mapping of thermostability of limit dextrinase in barley

journal contribution
posted on 2023-05-18, 10:42 authored by Wang, X, Zhang, X, Cai, S, Ye, L, Meixue ZhouMeixue Zhou, Chen, Z, Zhang, G, Dai, F
Limit dextrinase (LD) is an essential amylolytic enzyme for the complete degradation of starch, and it is closely associated with malt quality. A survey of 51 cultivated barley and 40 Tibetan wild barley genotypes showed a wide genetic diversity of LD activity and LD thermostability. Compared with cultivated barley, Tibetan wild barley showed lower LD activity and higher LD thermostability. A doubled haploid population composed of 496 DArT and 28 microsatellite markers was used for mapping Quantitative Trait Loci (QTLs). Parental line Yerong showed low LD activity and high LD thermostability, but Franklin exhibited high LD activity and low LD thermostability. Three QTLs associated with thermostable LD were identified. The major QTL is close to the LD gene on chromosome 7H. The two minor QTLs colocalized with previously reported QTLs determining malt-extract and diastatic power on chromosomes 1H and 2H, respectively. These QTLs may be useful for a better understanding of the genetic control of LD activity and LD thermostability in barley.

Funding

Grains Research & Development Corporation

History

Publication title

Journal of Agricultural and Food Chemistry

Volume

63

Issue

14

Pagination

3778-3783

ISSN

0021-8561

Department/School

Tasmanian Institute of Agriculture (TIA)

Publisher

Amer Chemical Soc

Place of publication

1155 16Th St, Nw, Washington, USA, Dc, 20036

Rights statement

© 2015 American Chemical Society

Repository Status

  • Restricted

Socio-economic Objectives

Barley

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