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Genome-Wide Association Study Using Extreme Truncate Selection Identifies Novel Genes Affecting Bone Mineral Density and Fracture Risk

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posted on 2023-05-17, 10:44 authored by Duncan, EL, Danoy, P, Kemp, JP, Leo, PJ, McCloskey, E, Nicholson, GC, Eastell, R, Prince, RL, Eisman, JA, Graeme JonesGraeme Jones, Sambrook, PN, Reid, IR, Dennison, EM, Wark, J, Richards, JB, Uitterlinden, AG, Spector, TD, Esapa, C, Cox, RD, Brown, SDM, Thakker, RV, Addison, KA, Bradbury, LA, Center, JR, Cooper, C, Cremin, C, Estrada, K, Felsenberg, D, Gluer, CC, Hadler, J, Henry, MJ, Hofman, A, Kotowicz, MA, Makovey, J, Nguyen, SC, Nguyen, TV, Pasco, JA, Pryce, K, Reid, DM, Rivadeneira, F, Roux, C, Stefansson, K, Styrkarsdottir, U, Thorleifsson, G, Tichawangana, R, Evans, DM, Brown, MA
Osteoporotic fracture is a major cause of morbidity and mortality worldwide. Low bone mineral density (BMD) is a major predisposing factor to fracture and is known to be highly heritable. Site-, gender-, and age-specific genetic effects on BMD are thought to be significant, but have largely not been considered in the design of genome-wide association studies (GWAS) of BMD to date. We report here a GWAS using a novel study design focusing on women of a specific age (postmenopausal women, age 55–85 years), with either extreme high or low hip BMD (age- and gender-adjusted BMD zscores of +1.5 to +4.0, n = 1055, or 24.0 to 21.5, n = 900), with replication in cohorts of women drawn from the general population (n = 20,898). The study replicates 21 of 26 known BMD–associated genes. Additionally, we report suggestive association of a further six new genetic associations in or around the genes CLCN7, GALNT3, IBSP, LTBP3, RSPO3, and SOX4, with replication in two independent datasets. A novel mouse model with a loss-of-function mutation in GALNT3 is also reported, which has high bone mass, supporting the involvement of this gene in BMD determination. In addition to identifying further genes associated with BMD, this study confirms the efficiency of extreme-truncate selection designs for quantitative trait association studies.

History

Publication title

PLoS Genetics

Volume

7

Issue

4

Article number

e1001372

Number

e1001372

Pagination

1-10

ISSN

1553-7390

Department/School

Menzies Institute for Medical Research

Publisher

Public Library of Science

Place of publication

United States

Rights statement

Licensed under Creative Commons Attribution 2.5 Generic (CC BY 2.5) http://creativecommons.org/licenses/by/2.5/

Repository Status

  • Open

Socio-economic Objectives

Clinical health not elsewhere classified

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