University Of Tasmania
ac89 11918-11923 Rahbar.pdf (1.49 MB)
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Geometrical alignment of multiple fabrication steps for rapid prototyping of microfluidic paper-based analytical devices

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journal contribution
posted on 2023-05-19, 14:28 authored by Rahbar, M, Nesterenko, PN, Brett PaullBrett Paull, Miroslav MackaMiroslav Macka
Three main fabrication steps for microfluidic paper-based analytical devices (μPADs) were fully integrated with accurate geometrical alignment between the individual steps in a simple and rapid manner. A wax printer for creating hydrophobic barriers was integrated with an inexpensive (ca. $300) electronic craft plotter/cutter for paper cutting, followed by colorimetric reagent deposition using technical pens. The principal shortcoming in the lack of accurate and precise alignment of the features created by these three individual fabrication steps was addressed in this work by developing appropriate alignment procedures during the multistep fabrication process. The wax printing step was geometrically aligned with the following cutting and plotting (deposition) steps in a highly accurate and precise manner using optical scanning function of the plotter/cutter based on registration marks printed on the paper using the wax printer and scanned by the plotter/cutter. The accuracy and precision of alignment in a two-dimensional plane were quantified by cutting and plotting cross-shaped features and measuring their center coordinates relative to wax printed reference lines. The average accuracy along the X- and Y-axis was 0.12 and 0.16 mm for cutting and 0.19 and 0.17 mm for plotting, respectively. The potential of this approach was demonstrated by fabricating μPADs for instrument-free determination of cobalt in waters using distance-based readout, with excellent precision (%RSD = 5.7) and detection limit (LOD) of 2.5 ng and 0.5 mg/L (mass and concentration LODs, respectively).


Publication title

Analytical Chemistry










School of Natural Sciences


Amer Chemical Soc

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1155 16Th St, Nw, Washington, USA, Dc, 20036

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© 2017 American Chemical Society

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