University of Tasmania
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Graphene Oxide-Functionalized Thread-Based Electrofluidic Approach for DNA Hybridization

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posted on 2023-11-03, 02:07 authored by Liang Wu, Arushi Manchanda, Vipul Gupta, Brett PaullBrett Paull
A novel, low-cost, and disposable thread-based electrofluidic analytical method employing isotachophoresis (ITP) was developed for demonstrating surface DNA hybridization. This approach was based on graphene oxide (GO) surface-functionalized zones on nylon threads as a binding platform to trap a fluorescently labeled isotachophoretically focused single-stranded DNA (ssDNA) band, resulting in quenching of the fluorescence, which signaled quantitative trapping. In the event of an isotachophoretically focused complementary DNA (cDNA) band passing over the GO-trapped ssDNA zone, surface hybridization of the ssDNA and cDNA to form double-stranded DNA (dsDNA) band occurred, which is released from the GO-coated zones, resulting in restoration of the fluorescent signal as it exits the GO band and migrates further along the thread. This controllable process demonstrates the potential of the GO-functionalized thread-based microfluidic analytical approach for DNA hybridization and its visualization, which could be adapted into point-of-care (POC) diagnostic devices for real-world applications.

History

Sub-type

  • Article

Publication title

ACS OMEGA

Medium

Electronic-eCollection

Volume

8

Issue

15

Pagination

13569-13577:9

eISSN

2470-1343

ISSN

2470-1343

Department/School

Chemistry

Publisher

AMER CHEMICAL SOC

Publication status

  • Published

Place of publication

United States

Event Venue

Australian Centre for Research on Separation Science (ACROSS) and ARC Centre of Excellence for Electromaterials Science (ACES), School of Natural Sciences (Chemistry), University of Tasmania, Hobart 7001, Tasmania, Australia.

Rights statement

Copyright © 2023 The Authors. Published by American Chemical Society. This publication is licensed under CC-BY-NC-ND 4.0.

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