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Ribeiro t al, Brazil paper 2023 in Plants.pdf (7.43 MB)

Hepatoprotective Effects of Four Brazilian Savanna Species on Acetaminophen-Induced Hepatotoxicity in HepG2 Cells

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posted on 2023-11-03, 05:12 authored by Gislane dos Santos Ribeiro, Diegue Henrique Nascimento Martins, João Victor Dutra Gomes, Noel DaviesNoel Davies, Christopher William Fagg, Luiz Alberto Simeoni, Mauricio Homem-de-Mello, Pérola Oliveira Magalhães, Dâmaris Silveira, Yris Maria Fonseca-Bazzo
We investigated four Cerrado plant species, i.e., Cheiloclinium cognatum (Miers) A.C.Sm, Guazuma ulmifolia Lam., Hancornia speciosa Gomes, and Hymenaea stigonocarpa Mart. ex Hayne, against acetaminophen toxicity using an in vitro assay with HepG2 cells. The activity against acetaminophen toxicity was evaluated using different protocols, i.e., pre-treatment, co-treatment, and post-treatment of the cells with acetaminophen and the plant extracts. HepG2 cell viability after treatment with acetaminophen was 39.61 ± 5.59% of viable cells. In the pre-treatment protocol, the extracts could perform protection with viability ranging from 50.02 ± 15.24% to 78.75 ± 5.61%, approaching the positive control silymarin with 75.83 ± 5.52%. In the post-treatment protocol, all extracts and silymarin failed to reverse the acetaminophen damage. In the co-treatment protocol, the extracts showed protection ranging from 50.92 ± 11.14% to 68.50 ± 9.75%, and silymarin showed 77.87 ± 4.26%, demonstrating that the aqueous extracts of the species also do not increase the toxic effect of acetaminophen. This protection observed in cell viability was accompanied by a decrease in ROS. The extracts' hepatoprotection can be related to antioxidant compounds, such as rutin and mangiferin, identified using HPLC-DAD and UPLC-MS/MS. The extracts were shown to protect HepG2 cells against future APAP toxicity and may be candidates for supplements that could be used to prevent liver damage. In the concomitant treatment using the extracts with APAP, it was demonstrated that the extracts do not present a synergistic toxicity effect, with no occurrence of potentiation of toxicity. The extracts showed considerable cytoprotective effects and important antioxidant characteristics.

History

Sub-type

  • Article

Publication title

Plants

Medium

Electronic

Volume

12

Issue

19

Pagination

3393

eISSN

2223-7747

ISSN

2223-7747

Department/School

Central Science Laboratory

Publisher

MDPI

Publication status

  • Published online

Place of publication

Switzerland

Event Venue

Pharmacy Department, Health Sciences School, University of Brasília, Brasilia 70910-900, Brazil.

Rights statement

Copyright 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/)