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High-throughput real-time PCR and melt curve analysis for sexing Southern Ocean seabirds using fecal samples

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posted on 2023-05-21, 05:39 authored by Faux, CE, Julie McInnesJulie McInnes, Jarman, SN

Sex identification of birds is of great interest in ecological studies, however this can be very difficult in many species because their external features are almost monomorphic between the sexes. Molecular methodology has simplified this process but limitations still occur with widely accepted methods using polymerase chain reaction and gel electrophoresis, especially when applied to degraded DNA. Real-time polymerase chain reaction assays are emerging as a more efficient, sensitive, and higher throughput means of identification, but there are very few techniques validated using fecal samples and small target sizes. We present a real-time melt curve analysis assay targeting a small region of the CHD-1 gene allowing for high-throughput, sensitive, specific, and easy-to-interpret sexing results for a variety of Southern Ocean seabirds using fecal and tissue samples.

History

Publication title

Theriogenology

Volume

81

Issue

6

Pagination

870-874

ISSN

0093-691X

Department/School

Institute for Marine and Antarctic Studies

Publisher

Elsevier Science Inc

Place of publication

360 Park Ave South, New York, USA, Ny, 10010-1710

Rights statement

Copyright 2014 The Authors. Published by Elsevier Inc. Open access under CC BY-NC-SA license. (https://creativecommons.org/licenses/by-nc-sa/3.0/)

Repository Status

  • Open

Socio-economic Objectives

Assessment and management of pelagic marine ecosystems; Marine biodiversity

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