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High-throughput sequencing of microbial communities in Poro cheese, an artisanal Mexican cheese

journal contribution
posted on 2023-05-18, 07:23 authored by Aldrete-Tapia, A, Escobar-Ramirez, MC, Mark TamplinMark Tamplin, Hernandez-Iturriaga, M
The bacterial diversity and structure of Poro cheese, an artisanal food, was analysed by high-throughput sequencing (454 pyrosequencing) in order to gain insight about changes in bacterial communities associated with the cheese-making process. Dairy samples consisting of milk, fermented whey, curd and ripened cheese (during 7 and 60 d) were collected from three manufacturers located in the state of Tabasco, México during dry (March–June) and rainy (August–November) seasons. Independently of producer and season, raw milk samples displayed the highest diversity in bacterial communities. In raw milk, genera found were Macrococcus, Staphylococcus, Enterococcus, Streptococcus, Lactobacillus and Enhydrobacter. Diversity in whey, curd and cheese was lower, principally containing Streptococcus and Lactobacillus; however, bacteria such as Staphylococcus, Acinetobacter, Chryseobacterium, Bacillus, Sediminibacter, Lactococcus and Enterococcus were occasionally present. After curdling step, the most dominant and abundant species were Streptococcus thermophilus and Lactobacillus delbrueckii.


Publication title

Food Microbiology








Tasmanian Institute of Agriculture (TIA)


Academic Press Ltd Elsevier Science Ltd

Place of publication

24-28 Oval Rd, London, England, Nw1 7Dx

Rights statement

Copyright 2014 Elsevier Ltd.

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Socio-economic Objectives

Food safety

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