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Human brain beta-A4 amyloid protein precursor of Alzheimer's disease: purification and partial characterization.
journal contribution
posted on 2023-05-17, 10:05 authored by Moir, RD, Martins, RN, David SmallDavid Small, Bush, AI, Milward, EA, Multhaup, G, Beyreuther, K, Masters, CLThe major component of the amyloid deposition that characterizes Alzheimer's disease is the 4-kDa βA4 protein, which is derived from a much larger amyloid protein precursor (APP). A procedure for the complete purification of APP from human brain is described. The same amino terminal sequence of APP was found in two patients with Alzheimer's disease and one control subject. Two major forms of APP were identified in human brain with apparent molecular masses of 100–110 kDa and 120–130 kDa. Soluble and membrane fractions of brain contained nearly equal amounts of APP in both humans and rats. Immunoprecipitation with carboxyl terminus-directed antibodies indicates that the soluble forms of APP are truncated. Carboxyl terminus truncation of membrane-associated forms of human brain APP was also found to occur during postmortem autolysis. The availability of purified human brain APP will facilitate the investigation of its normal function and the events that lead to its abnormal cleavage in patients with Alzheimer's disease.
History
Publication title
Journal of NeurochemistryVolume
59Issue
4Pagination
1490-1498ISSN
0022-3042Department/School
Menzies Institute for Medical ResearchPublisher
Blackwell Publishing LtdPlace of publication
9600 Garsington Rd, Oxford, England, Oxon, Ox4 2DgRights statement
The definitive published version is available online at: http://www3.interscience.wiley.com/Repository Status
- Restricted