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Hydrogen/deuterium exchange on aromatic rings during atmospheric pressure chemical ionization mass spectrometry

journal contribution
posted on 2023-05-17, 01:18 authored by Noel DaviesNoel Davies, Jason SmithJason Smith, Molesworth, PP, John RossJohn Ross
It was demonstrated that substituted indoles fully labelled with deuterium on the aromatic ring can undergo substantial exchange back to partial and even fully protonated forms during Atmospheric Pressure Chemical Ionisation (APCI) LC-MS. The degree of this exchange was strongly dependent on the absolute quantity of analyte, the APCI desolvation temperature, the nature of the mobile phase, the mobile phase flow rate and the instrument used. H-D exchange on several other aromatic ring systems during APCI LC-MS was either undetectable (nitrobenzene, aniline) or extremely small (acetanilide) compared to the effect observed for substituted indoles. This observation has major implications for quantitative assays using deuterium labelled internal standards and for the detection of deuterium labelled products from isotopically labelled feeding experiments where there is a risk of back exchange to the protonated form during the analysis.

History

Publication title

Rapid Communications in Mass Spectrometry

Volume

24

Issue

7

Pagination

1105-1110

ISSN

1097-0231

Department/School

School of Natural Sciences

Publisher

John Wiley & Sons

Place of publication

New York

Rights statement

The definitive published version is available online at: http://interscience.wiley.com

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the chemical sciences

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