IgM ANCA in healthy individuals and in patients with ANCA-associated vasculitis
journal contribution
posted on 2023-05-20, 06:07authored byJeffs, LS, Peh, CA, Nelson, A, Tan, PG, Davey, E, Katherine ChappellKatherine Chappell, Perkins, GB, Hurtado, PR
Low levels of IgM auto-antibodies have been reported in health and disease. IgM anti-neutrophil cytoplasmic antibodies (ANCA) have been reported in patients with ANCA-associated vasculitis (AAV). We sought to investigate if healthy individuals may have IgM ANCA in their sera. The first aim of the study was to determine whether IgM ANCA was present in healthy individuals and in patients with ANCA-associated vasculitis. The second aim was to determine what happens to IgM ANCA levels over time. The third aim was to determine whether bacterial infections affected IgM ANCA levels in non-AAV patients. Sera from healthy individuals and patients with AAV were tested for IgM ANCA by immunofluorescence on fixed neutrophils, immunoprecipitation, Western blot and ELISA. Peripheral blood mononuclear cells were isolated and tested by ELISpot for circulating IgM ANCA B cells. To determine whether infection affected IgM ANCA levels, we studied non-AAV patients with bacterial endocarditis or Staphylococcus aureus bacteraemia and measured IgM ANCA levels over time. IgM ANCA is detectable in both healthy individuals and patients with AAV and the titres decreased with increasing age. Circulating IgM ANCA B cells were identified by ELISpot. In the presence of infection, we could not find a significant change in IgM ANCA levels. We report the presence of low-level specific IgM ANCA in the sera of healthy individuals and in patients with ANCA-associated vasculitis. Bacterial infection did not affect the level of IgM ANCA in this small study.
History
Publication title
Immunologic Research
Volume
67
Pagination
325-336
ISSN
0257-277X
Department/School
Menzies Institute for Medical Research
Publisher
Humana Press Inc
Place of publication
999 Riverview Drive Suite 208, Totowa, USA, Nj, 07512
Rights statement
Copyright Springer Science+Business Media, LLC, part of Springer Nature 2019