University of Tasmania
Browse

File(s) under permanent embargo

Impacts of Fsh, Igf1, and high temperature on the expression of genes involved in steroidogenesis, cell communication, and apoptosis in isolated coho salmon previtellogenic ovarian follicles

journal contribution
posted on 2023-05-20, 02:00 authored by Kelli AndersonKelli Anderson, Luckenbach, JA, Yamamoto, Y, Elizur, A
In salmonids, exposure to elevated temperature impairs oogenesis. As such, there is a need to understand the molecular mechanisms that underpin this process, and develop mitigation strategies that maintain or rescue reproductive development in broodstock. In this study, follicle stimulating hormone (Fsh) and/or insulin-like growth factor 1 (Igf1) treatment were assessed for their ability to promote reproductive function at 14 and 22 °C in ovarian follicles from coho salmon in vitro. Maintenance at 22 °C resulted in the downregulation of fsh receptor, 17α-hydroxylase/C17,20-lyase and p450 aromatase a (cyp19a1a), and connexin 34.3 (cx34.3). While combined treatment with Fsh and Igf1 stimulated the expression of cyp19a1a at 14 °C, this treatment was not effective at 22 °C. Upregulation of cx34.3 occurred in response to treatments that contained Igf1 regardless of temperature, and there is evidence to suggest that apoptosis was inhibited to some extent at 22 °C following combined treatment with Fsh and Igf1. This study demonstrates the thermal impairment of key reproductive genes, and highlights the potential for novel hormone treatments to rescue oogenesis in salmonids.

History

Publication title

Aquaculture

Volume

506

Pagination

60-69

ISSN

0044-8486

Department/School

Institute for Marine and Antarctic Studies

Publisher

Elsevier Science Bv

Place of publication

Po Box 211, Amsterdam, Netherlands, 1000 Ae

Rights statement

© 2019 Elsevier B.V.

Repository Status

  • Restricted

Socio-economic Objectives

Aquaculture fin fish (excl. tuna); Expanding knowledge in the biological sciences

Usage metrics

    University Of Tasmania

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC