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Download fileIndirect Photometric Detection in CE Using Buffered Electrolytes - Part 1, Principles
journal contribution
posted on 2023-05-16, 13:01 authored by Miroslav MackaMiroslav Macka, Johns, CA, Doble, PA, Paul HaddadPaul HaddadIndirect photometric detection in capillary electrophoresis is valuable as a detection method for nonabsorbing low molecular weight ions. The general operating principle rests in the displacement of an absorbing ion — sometimes called the probe — added to the electrolyte by the analyte co-ions, which are ions of the same charge polarity, as they migrate under the influence of the electric field through the capillary. Despite this apparent simplicity, the choice of optimal parameters, and especially of electrolyte composition, can be quite challenging. This first installment of a two-part series outlines the principles and approaches to indirect detection.
History
Publication title
LC GC North AmericaVolume
19Pagination
38-47ISSN
1527-5949Department/School
School of Natural SciencesPublisher
Advanstar CommunicationsPlace of publication
Duluth, MN, USARights statement
Copyright © 2001 Advanstar Communications Inc.Repository Status
- Open