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Integrated modeling of survival data from multiple stressor ecotoxicology experiments

journal contribution
posted on 2023-05-19, 14:52 authored by Proctor, AH, King, CK, Holan, JR, Wotherspoon, SJ
Ecotoxicological assessments often focus on the response of an organism to an individual contaminant under standardized laboratory conditions. Under more ecologically realistic conditions, however, individuals are likely to be exposed to a range of environmental conditions that have the potential to act as additional stressors. Multiple-stressor experiments improve our understanding of an organism’s response to a toxicant under ecologically relevant conditions and provide realistic risk assessment data. To date, there is no standardized method for analyzing multiple-stressor data using dose-response regression. We present a reliable technique to assess for the effects of additional stressors on an LCx estimate in a consistent framework, providing interpretable results that meaningfully deal with environmental changes and their possible impacts on sensitivity estimates to a toxicant. The method is applicable to any data set where toxicity tests are conducted at varying levels of one or more additional stressors. We illustrate the method with data from an experiment that investigates the effects of salinity and temperature on the sensitivity of the subantarctic isopod Limnoria stephenseni to copper, where it is shown that the major change in the LC50 can be primarily attributed to a specific temperature increase. This method has been incorporated into an R package available at


Publication title

Environmental Science and Technology










Institute for Marine and Antarctic Studies


Amer Chemical Soc

Place of publication

1155 16Th St, Nw, Washington, USA, Dc, 20036

Rights statement

Copyright 2017 American Chemical Society

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the environmental sciences

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