University of Tasmania
Browse

Integration of transcriptome and metabolome analyses reveals the mechanistic basis for cadmium accumulation in maize

Download (3.41 MB)
journal contribution
posted on 2023-05-21, 15:01 authored by Lin, K, Zeng, M, Williams, DV, Hu, W, Sergey ShabalaSergey Shabala, Meixue ZhouMeixue Zhou, Coa, F
Cadmium (Cd) pollution in soil has become a major environmental issue worldwide. However, the underlying molecular mechanism of low grain-Cd accumulation (GCA) in maize is still largely unknown. Herein, we report the mechanistic basis for low GCA in maize by a multiomics approach. The low GCA genotype L63 showed normal vacuolar formation and a lower capacity of xylem loading of Cd than the high-accumulator L42 under Cd stress. Transcriptomic sequencing identified 84 low-GCA-associated genes which are mainly involved in the S-adenosylmethionine (SAM) cycle, metal transport, and vacuolar sequestration. A metabolome analysis revealed that L63 plants had a more active SAM cycle and a greater capacity for terpenoid synthesis and phenylalanine metabolism than L42. Combining the analysis of transcriptome and metabolome characterized several genes as key genes involved in the determination of Cd accumulation. Our study identifies a mechanistic basis for low Cd accumulation in maize grains and provides candidate genes for genetic improvement of crops.

History

Publication title

iScience

Volume

25

Issue

12

Article number

105484

Number

105484

Pagination

1-17

ISSN

2589-0042

Department/School

Tasmanian Institute of Agriculture (TIA)

Publisher

Cell Press

Place of publication

United States

Rights statement

© 2022 The Author(s). This is an open access article under the Attribution-Non Commercial-No Derivatives 4.0 International(CC BY-NC-ND) license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Repository Status

  • Open

Socio-economic Objectives

Wheat

Usage metrics

    University Of Tasmania

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC